Bead Deposition

 3'-end Modification

The templated beads are 3’ modified in order to facilitate a covalent linkage to the slide.

3'-end modification

Bead Deposition

 After 3' modification, the beads deposited on a glass slide in a random array. Different deposition chambers can segment a slide into one, four or eight chambers, and consequently different samples can be loaded in the same sequence run. In general, the one, four and eight chambers slide can hold 165 million, 30 million(per individual well) and 14 million(per individual well) Million of P2-Positive Beads, respectively. 

beads deposited